No amplification of SSR markers on Pecan nuts project?

Hi all, I am doing an SSR project on Pecan nuts and I am struggling to get amplification on my PCR. Here is a summary of what I have done so far 1. DNA extraction was a modified CTAB protocol...

19 April 2020 8,678 2 View

By how much does the relative response factor of two isomers differ?

08 February 2017 2,178 3 View

During the RNA precipitation step with isopropanol during a TriZol extraction, can glycogen carry contaminants into the RNA pellet?

I have been doing TriZol RNA extractions of zebrafish embryos with and without glycogen as a carrier. I've found that using glycogen during the isopropanol precipitation step increases the size...

15 August 2016 6,758 6 View

Hi all, could anyone give me some advice on transformation via electroporation?

23 November 2014 3,510 3 View

What is the point of switching cells to a 2% FBS-containing media before analyzing Ras-Raf signaling?

We are treating cancer cells with inhibitors to see if we can inhibit the Ras-Raf interaction. I can clearly detect the interaction via coIP but I am having trouble seeing inhibition of this...

09 October 2014 1,982 6 View

Can fMRI be used to demonstrate telepathy?

I have an idea for an experiment requiring fMRI, which would demonstrate the existence of mirroring neuronal activity in the absence of visual or auditory stimuli. I would be interested in...

29 June 2014 5,990 6 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

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01 March 2021 3,905 5 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View

Dissertation Topic Ideas for MSc Business Management?

Hello, I hope everyone reading this is doing well during these tough times. I am doing my masters in business management and considering dissertation topics. I'm interested in taking an...

15 February 2021 594 26 View

How do I group questions of SPSS under one variable for Spearman's r?

12 February 2021 5,379 5 View

Where can I find the Affiliate Stigma Scale developed by Mak and Cheung?

Need the scale for a dissertation. Would be better if I could get it for free since my work is not funded. I need the English version of this scale.

09 February 2021 4,022 3 View