Free FACS software

More David Pla-Martín's questions See All

How to analyze properly images from microscopy?

Hello! I have a question regarding the quantification of data obtained from microscopy. It is clear that if you take several images from the same organ/tissue, you can not consider one picture as...

10 November 2016 1,671 4 View

Attaching acute slices for confocal imaging

Hi again: I am trying to optimize a protocol for imaging calcium dynamics in a cell population in the dentate gyrus and am facing some trouble. I made a similar question and got 1 answer but I...

05 June 2016 10,183 2 View

How can I increase ATP levels in mice?

Hello everybody, I wonder if it is possible to increase ATP levels in mice just giving them water with Glucose in order to push glycolysis. I am working with a liver KO model where OXPHOS seems to...

04 May 2015 294 9 View

Which is a good Tissue Cell Lysis Buffer for Luminescent ATP assay?

Hello everybody. I would like to do measurements of ATP in pieces of liver from a KO mouse. I was wondering if anyone of you know a good buffer to solubilize liver pieces and then proceed with a...

01 February 2015 9,623 7 View

How to nucleofect SH-SY5Y?

I am trying to transfect SH-SY5Y cells with two plasmids with a high efficient method. I have decided to use Lonza nucleofector in order to obtain better results than using FuGene or...

01 February 2014 7,489 3 View

Does anyone know how to quench Fura-2 AM fluorescence?

I am recording changes in cytosolic calcium using Fura-2 AM in cells previously transfected with a GFP tagged protein. In order to obtain fluorescence background added by GFP I am trying to quench...

03 April 2013 5,682 13 View

How do you explain that the same sample gives different results on gene expression analysis by qPCR?

I have been using cDNA from 1ug of total RNA isolated with trizol. Also taking care with RNAse

02 March 2013 7,595 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Christina Stoeckle

Our colleagues with Macs use WEASEL and are happy with it: http://www.wehi.edu.au/faculty/advanced_research_technologies/flow_cytometry/weasel_for_flow_cytometry_data_analysis

Ishak Ozel Tekin

http://www.cyto.purdue.edu/flowcyt/software.htm

Hessah Sulami

Flowjo is good and you can use it with mac.

http://www.flowjo.com/download-for-mac/