How to analyze properly images from microscopy?

Hello! I have a question regarding the quantification of data obtained from microscopy. It is clear that if you take several images from the same organ/tissue, you can not consider one picture as...

10 November 2016 1,514 4 View

Attaching acute slices for confocal imaging

Hi again: I am trying to optimize a protocol for imaging calcium dynamics in a cell population in the dentate gyrus and am facing some trouble. I made a similar question and got 1 answer but I...

05 June 2016 10,007 2 View

How can I increase ATP levels in mice?

Hello everybody, I wonder if it is possible to increase ATP levels in mice just giving them water with Glucose in order to push glycolysis. I am working with a liver KO model where OXPHOS seems to...

04 May 2015 183 9 View

Which is a good Tissue Cell Lysis Buffer for Luminescent ATP assay?

Hello everybody. I would like to do measurements of ATP in pieces of liver from a KO mouse. I was wondering if anyone of you know a good buffer to solubilize liver pieces and then proceed with a...

01 February 2015 9,451 7 View

How to nucleofect SH-SY5Y?

I am trying to transfect SH-SY5Y cells with two plasmids with a high efficient method. I have decided to use Lonza nucleofector in order to obtain better results than using FuGene or...

01 February 2014 7,366 3 View

Does anyone know how to quench Fura-2 AM fluorescence?

I am recording changes in cytosolic calcium using Fura-2 AM in cells previously transfected with a GFP tagged protein. In order to obtain fluorescence background added by GFP I am trying to quench...

03 April 2013 5,554 13 View

How do you explain that the same sample gives different results on gene expression analysis by qPCR?

I have been using cDNA from 1ug of total RNA isolated with trizol. Also taking care with RNAse

02 March 2013 7,481 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View