David Pla-Martín

7 Questions 18 Answers 0 Followers

Questions related from David Pla-Martín

How to analyze properly images from microscopy?

Hello! I have a question regarding the quantification of data obtained from microscopy. It is clear that if you take several images from the same organ/tissue, you can not consider one picture as...

11 November 2016 1,628 4 View

How can I increase ATP levels in mice?

Hello everybody, I wonder if it is possible to increase ATP levels in mice just giving them water with Glucose in order to push glycolysis. I am working with a liver KO model where OXPHOS seems to...

05 May 2015 268 9 View

Which is a good Tissue Cell Lysis Buffer for Luminescent ATP assay?

Hello everybody. I would like to do measurements of ATP in pieces of liver from a KO mouse. I was wondering if anyone of you know a good buffer to solubilize liver pieces and then proceed with a...

02 February 2015 9,584 7 View

Free FACS software

Can anyone suggest a free software for Mac to open an analyze FACS files obtained in FACS Diva (.fcs)?

09 September 2014 8,790 3 View

How to nucleofect SH-SY5Y?

I am trying to transfect SH-SY5Y cells with two plasmids with a high efficient method. I have decided to use Lonza nucleofector in order to obtain better results than using FuGene or...

02 February 2014 7,455 3 View

Does anyone know how to quench Fura-2 AM fluorescence?

I am recording changes in cytosolic calcium using Fura-2 AM in cells previously transfected with a GFP tagged protein. In order to obtain fluorescence background added by GFP I am trying to quench...

04 April 2013 5,648 13 View

How do you explain that the same sample gives different results on gene expression analysis by qPCR?

I have been using cDNA from 1ug of total RNA isolated with trizol. Also taking care with RNAse

03 March 2013 7,562 2 View