Is there any labs in India working with Brachypodium distachyon? Do anyone have its seeds ?

29 January 2016 469 3 View

Can someone suggest how to avoid the Protein A/G bead detection in western blot after Immunoprecipitation?

I am using Polyclonal antibody form rabbit for IP and Polyclonal anti GFP and Anti rabbit HRP for western. I have tried in the reverse way also but still i am getting high background of Protein...

23 July 2013 6,349 7 View

Problem in Ponceau staining after protein Transfer

Very recently I noticed that even if I have enough protein on the gel, enough to be detected by commassie stain- I do not get it stained by Ponceau on the blot after transfer. The protein...

18 October 2012 8,312 17 View

How to segment 3D cone beam CT images in MatLab? Are there ways for segmentation of 3D CBCT images?

I want to segment 3D cone beam CT images and haven't found good methods to solve my questions. The volume of 3D CBCT image is consisted of many 2D slice images.

28 September 2012 7,083 11 View

Cloud server storage security - Mobile cloud environment

Opening the forum for scholars to collaborate and work on the field of cloud storage security. Interested at hers can ping to collaborate and bring out more secure solutions

01 January 1970 6,580 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

How we can dissolve theophylline derivatives for the MTT test?

I have a question about your letter, Fluorescent phosphoinositide 3-kinase inhibitors suitable for monitoring of intracellular distribution. Can you tell me how we can dissolve theophylline for...

26 January 2021 9,094 1 View