Found a plant extract found it to be a strong mitotic inhibitor. How to proceed further with this ?

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How to extract good quality RNA from Brown Algae?

We have been trying to extract RNA from brown algae. We have been following traditional CTAB method as modified by Sim et al., 2013. We get a very thick white jelly interface with the Chloroform...

30 May 2024 356 0 View

How can I resolve peak tailing during the analysis of drug in HPLC?

I would like to resolve peak tailing in the hplc analysis of one drug. Mobile phase used is 100% Methanol. The sample injected after saponification. The sample pKa value is 16.5 before...

18 September 2021 731 3 View

When do we see very thick 5s rRNA band and faint 28s and 18s rRNA for yeast total RNA isolation?

I am trying to isolate total RNA from yeast lysate. The lysis is done using glass-bead beating. The lysate is collected and precipitated overnight with sodium acetate and ethanol. The precipitant...

30 July 2021 3,439 2 View

How are normalized tag densities calculated in MNase seq data?

I am very new to NGS analysis and will be analysing MNase data soon. MNase is used to identify nucleosome positions genome wide. I read papers and observed that normalized tag densities are used....

10 May 2020 5,621 0 View

Can somebody help me in getting a blog where I can find list of special issues(Elsevier/SCI indexed) in mechanical engineering stream ?

Looking for thermal engineering stream

09 June 2019 3,287 0 View

Why is polyethylene glycol (PEG) used for the preparation of competent yeast cells? Does it affect the cellular osmolarity of yeasts?

Competent yeast cell preparation makes use of PEG which has been implicated in bringing the cells and the plasmid in close proximity for effective transformation. Is there any proper evidence for...

20 June 2017 9,395 24 View

Universal procol for deriving callus from monocot leaf explants?

Is there any universal protocol for deriving callus from monocot plants?

14 March 2016 3,951 1 View

Will seed derived callus sustain desiccation tolerance just like that of seed?

I want to know whether callus derived from seeds can sustain desiccation tolerance just like that of seed?

18 February 2016 4,362 5 View

Is there any labs in India working with Brachypodium distachyon? Do anyone have its seeds ?

Need a model monocot plant. If possible Brachypodium distachyon seeds.

29 January 2016 701 3 View

Could you please describe, what is the difference between intracellular and cytoplasm localization?

Hi I am using confocal microscopy to analyze localization of Arabidopsis proteins. I couldn't understand the notions between intracellular and cytoplasmic (I reckon both can be called cytoplasmic).

20 July 2014 5,132 3 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

What is the method of standard curve of IAA preparation?

Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...

29 June 2024 4,421 1 View

Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

23 June 2024 2,287 7 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

05 June 2024 6,447 1 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

How does Post-Intensive Care Syndrome (PICS) impact the lives of ICU survivors in the long term?

Navigating the Post-ICU Journey: A Holistic Approach to Reco... How does Post-Intensive Care Syndrome (PICS) impact the lives of ICU survivors in the long term?

25 May 2024 4,161 1 View