Mono- species biofilm such as E.faecalis
Dual - species biofilm such as E.faecalis & S.mutans
What is better for intracanal medicamments antibacterial experiment?
Hello Nawras!
You know in nature it is pretty rare to find pure bacterial colonies made up of one species. Microbes, along with other organisms dwell and interact at all different levels. In fact, over 100 bacterial taxa can be isolated from root canal infections mostly G -ve anaerobes, in addition to yeasts!
This paper Article A critical analysis of research methods and experimental mod...
summarises a number of methodologies, highlighting your particular question about the number of organisms.
These papers may also answer your question:
1. https://pubmed.ncbi.nlm.nih.gov/22251411/
2. https://pubmed.ncbi.nlm.nih.gov/22000451/
3. Article The mycobiome of root canal infections is correlated to the bacteriome
However, if you have the resources and time, you may try both models and compare your findings!
Good luck
For testing the antibacterial effect of intracanal medicaments, its better to use mono-species biofilm such as E. Faecalis.
It is better to use mono-species biofilm for testing antibacterial effect
Endodontic root canal space harbours mainly obligate anaerobes. Studies have shown that mixed anaerobes mainly obligate followed by porphyromonas, streptococcus, fusobacterium, prevotella, e faecalis, bacteriodes, candida..these are the mainly seen microflora..so better go for a broad range for testing
Hi, defenitly mono species, because it is more acurate than dual species.
Endodontic biofilm is comprised of multispecies microorganisms with majority of obligate anaerobic species.
For antibacterial experiments of root canal medicaments, it is better to use biofilm to evaluate its effectiveness
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