For synthetic biology, what would be a good transcriptional activator domain in bacteria, similar to VP64 in mammalian cells?

01 December 2015 1 9K Report

Good properties of that domain would be, that it can be tagged to DNA-binding proteins. It should also induce robust expression of the gene that the DNA-binding protein is bound to.

Thanks for the help!

Dong-Jiunn Jeffery Truong Zhang

Short answer: Alpha or Omega subunit of the RNA polymerase. Attached are two papers regarding alpha subunit.

Article Identifying DNA sequences recognized by a transcription fact...

Article A bacterial two-hybrid selection system for studying protein...

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