I haven't draw the growth curve to know the doubling time.
Hello Soheila,
with "unknown" cells you can simply go by surface area, i.e. split ratio. Usually MCF7 should grow quite rapidly and you can pass on 1/6 of your cell suspension to a new flask of the same surface area. If you're not sure you can do 1:2, 1:4, 1:6 and see what works best for you. If I remember right they do not detach immediately after reaching confluence.
Hope that helps..
Thanks thomas,
So there is no need to count them every time, right?
By the way does MCF7 need a high glucose(4.5 g/ml) or low glucose(1 g/ml) DMEM?
i do appreciate you.
Hi Soheila,
So there is no need to count them every time, right?
Absolutely, Yes.
good luck
Hello Soheila,
right, you do not need to count them for nomal passing. Counting is important of you plate the cells for an assay, like western blot, FACS analysis, proliferation and so on, to be sure to always work with the same numbers, i.e. cell densities.
We had MCF7 growing in Leibovitz L15, which has 0.9g galactose. I guess they will grow in normal DMEM, but may prefer/grow faster in HG DMEM, it will probably not harm them.
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