For sequence alignment is it necessary to align the forward and reverse sequence of the same gene?

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How do you upload sequences in NCBI and what is the procedure?

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Could you try using PeptiCloud and see if it's a useful tool for biology research?

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Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

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Sourav Nayak

check out this discussion:

https://www.researchgate.net/post/Why_paired-end_reads_are_typically_selected_in_de_novo_assembly2

Diego Diez

Do you mean sequence alignment as on aligning different sequences from homolog genes? Or is sequence alignment related to NGS? If the first thing, then you do not need to align forward and reverse sequences at all...