How to identify the proper marker gene from plant for DNA Barcoding?

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Martin Devonshire

Six loci, matK, rbcL, rpoB, rpoC1, trnH-psbA spacer from the chloroplast genome and ITS are generally used for plant barcoding...

Ana Morais Pinela

Hi, if you are interested in information on the DNA barcoding visit their website (http://www.barcodeoflife.org/content/about/what-dna-barcoding), and take a closer look at the document CBOL's Data Standards, it is very explanatory. In this document it says that for plants:

"...ii) In November 2009, CBOL approved rbcL and matK as the barcode regions for vascular plants. They are defined relative to the Arabidopsis thaliana chloroplast NC_000932 sequence annotation as follows: the rbcL barcode region is at the 5' end of the rbcL gene between bp1-599 (27-579 excluding primer sequences); the matK

barcode region is between bp205-1046 (227-1019 excluding primer sequences)..."

Hope this can help, good luck!

Best regards,

Ana