For sequence alignment is it necessary to align the forward and reverse sequence of the same gene?

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Sourav Nayak

check out this discussion:

https://www.researchgate.net/post/Why_paired-end_reads_are_typically_selected_in_de_novo_assembly2

Diego Diez

Do you mean sequence alignment as on aligning different sequences from homolog genes? Or is sequence alignment related to NGS? If the first thing, then you do not need to align forward and reverse sequences at all...