Good day house.
I am conducting a research on hemoparasites like plasmodium, hemoproteus, leucocytozoon (Phylum Apicomplexans). Before running my PCR, I intend to check my PCR primers using in silico PCR in the UCSC Genome browser. But I can't find any genome for protozoans/parasites, rather only genomes for the hosts of these parasites are there - homo sapiens, megabats, microbats etc.
Please how do I go about this? I mean which genome can I use on the UCSC genome browser to conduct in silico PCR for protozoans/parasites given that their genomes are not listed?
Secondly, what tools can I use to assess my PCR primers? I mean tools that will easily tell me whether there are hairpins in my primers and possibility of self dimer?
Thanks.
Insilico PCR on UCSC genome browser gave me 100 percent match of primers but next day giving no match?
I just saw your message. Please check with if the assembly is same as you used previously
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