23 Questions 13 Answers 0 Followers
Questions related from Olayinka Sunday Okoh
Good day scholars. Please I need to determine the dopamine level in the brains 🧠 of experimental rats 🐀. I don't want to use ELISA; the alternative method I got requires spectroflurimeter which I...
11 June 2022 6,738 0 View
Good day scholars. Please can anyone help with detailed protocol(s) for the determination of Beta-Amyloid, dopamine, Nitric oxide (NO), Monoamine oxygenase A (MAO-A), and Acetylcholine esterase...
09 August 2021 2,335 1 View
Good day scholars. Please I need to measure the blood pressure of rats at interval. I will appreciate if anyone can help with link to get this tail cuff/monitor?📱
26 July 2021 8,382 2 View
Good day scholars. I am supposed to centrifuge my tissue homogenate at 10,000 rpm for 15 minutes. But the rotor head I have can't go beyond 5,000 rpm. How do I determine the duration of...
29 June 2021 744 3 View
Good day scholars. I am not able to get some Sigma products for biochemical assays. The supplier said alternative available products are from CDH and Loba-Chemie. But people hardly refer to these...
16 June 2021 5,978 3 View
Good day scholars. Please I need a step by step guide on how to collect vaginal smear and examine it for the presence of sperm. This is just to identify Pregnancy Day 1 when I am to start dosing...
31 May 2021 2,717 4 View
Good day great scholars. Please I would appreciate help on how to install Discovery Studio and PyRx in Ubuntu. I will also like to know how I can assess/identify the path of the License Pack for...
27 May 2021 9,773 10 View
Good day house. Please is there an acceptable way of inducing Lesch-Nyhan disease in rats? Administering 6-OHDA (6 hydroxydopamine) is just to disrupt neurotransmitters levels/ neurological...
12 May 2021 5,506 0 View
Good day scholars. Please I need detail protocol on how to isolate dithymoquinone from black seeds. I intended to buy dithymoquinone directly but what I kept getting is thymoquinone. Hence,...
03 December 2020 4,157 1 View
Thymoquinone and Dithymoquinone are found in black seeds (Nigella sativa). From their structures, dithymoquinone has 2 rings while thymoquinone has 1 ring. One would expect that dithymoquinone...
03 December 2020 410 2 View
Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...
16 October 2020 7,614 4 View
Good day house. Please can I plot Bar Chart with significance levels represented by alphabets in R? A file is attached to show the graphical representation of my question. I came across this bar...
16 October 2020 7,395 4 View
From my experiment, I determined MDA, AST, ALT, SOD, T. Cholesterol and HDL Cholesterol in various organs Plasma, Liver, Kidney, and Heart. To get their relationships in each of the organ, I drew...
23 May 2020 7,134 0 View
Good day house. Please is there R package and code to calculate MEAN +/- SEM? I know of MEAN +/- SD, but I have not come across MEAN +/- SEM. Besides, is it possible to generate data set given...
06 April 2020 3,966 3 View
Good day house. Please I need the assistance of anyone good in the use of R for data analysis. I have six groups (1 - 6) which are Factors i.e Categorical variables and each of these 6 groups...
06 April 2020 1,290 0 View
Good day house. I am conducting a research on hemoparasites like plasmodium, hemoproteus, leucocytozoon (Phylum Apicomplexans). Before running my PCR, I intend to check my PCR primers using in...
24 April 2019 2,374 2 View
I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...
12 April 2019 5,642 3 View
Good day house. I am working on Molecular Evolution and Phylogenetics of some organisms. Before conducting my Synonymous to Non Synonymous substitutions after Sanger Sequencing, I would need to...
09 April 2019 5,133 3 View
Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...
13 March 2019 6,801 4 View
I need to run PCR using various published primers. For one of the primers I intend to use BJ1 & BN2. For this primer a paper reported using 2.5ul of 10x PCR buffer containing 15mM MgCl2...
11 March 2019 9,452 6 View
Good day house. I used R for some statistical analysis. I will like to know how to specifically cite it because from the citation given by 'R Core Team' the publication is a manual, not a book,...
28 February 2019 4,188 3 View
Good day house. Please I need to use command line (like wget, ) to retrieve nucleotide/amino acid sequences of giardia Thioredoxin reductase from databases like NCBI GenBank, eupathdb and...
11 November 2018 4,374 2 View
I want to commpare the diversity and prevalence of some blood parasites/hemoprotozoan parasites, ectoparasites and endoparasites in bats. I will be using two different locations (campuses) in...
08 July 2017 5,335 3 View
