For in silico analysis of a variant, howmany bioinformatic tools are required to give a sign in the favour of its pathogenecity?

Dear fellows, I am wondering about, how many bioinformatic tools are required to declare a variant as pathogenic and deleterious?

02 October 2020 6,580 3 View

Plasmid visualization on gel electrophoresis?

I extract the plasmid from e. Coli, on nano drop its concentration was 60.but no band was formed in gel electrophoresis?I use 1% agrose gel with 2.5 ethidum bromid.

06 September 2020 4,438 11 View

Applying Plant extracts on cell line?

Has anybody tried applying plant extracts on MCF 7 cell lines after filtering them? will it (filtering) effect the antioxidant activity present in plants?

12 March 2020 5,258 3 View

No title

02 June 2019 8,134 2 View

Given in 3D Ellipsoid semi major axis a=5 and b =4 ,c=3 . How can we find the distance D between two focal points (P0 and P2)?

Given in 3D Ellipsoid semi major axis a=5 (along axis) and semi minor axis b =4 ,c=3 (along y and z axis) . How can we find the distance D between two focal points (P0 and P2) ? Also how to find...

09 May 2019 8,093 4 View

How to fix the range of 't' (time) in matlab such that the delta function returns the impulse response for each dealy in matlab?

How to fix the range of 't' (time) in matlab such that the delta function returns the impulse response for each dealy in matlab. Can anyone help me in this regard ? I am having problem setting...

04 May 2019 8,787 3 View

Does surface doping always develop hetrojunction at the interface?

Is surface doping like attachment of noble or other metals at the exterior of nanomaterials? Hetrojunctions are developed in the second case, but dopant is in very small concentration ,would it...

11 May 2018 868 4 View

How the bacteria can be differentiated visually in a mixed culture by adding aniline blue to MRS medium?

I am using mixed culture of LAB and Yeast for cereal fermentation.For enumeration of each bacterium in a mixed culture, I need to add anilin blue to the MRS medium but I didn't found how the...

23 July 2017 9,525 23 View

How to prepare sample for Photoluminescence , if it is insoluble in water?

PL technique requires liquid sample. Can it be used as a suspension for water insoluble materials?

01 May 2017 5,646 3 View

Why we use DNAses in RNA isolation as in end for PCR analysis we have to finally convert RNA to DNA.

08 February 2017 9,218 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View