CDNA synthesis or qPCR did not work?

Hi everyone! I extracted RNA from urine pellet using phenol-chloroform method. The yield of RNA was 160ng/uL. 3uL of RNA was used for cDNA synthesis in 20 uL reaction using Go-Script kit....

05 February 2021 6,587 4 View

How to improve the techniques for gel picture of RNA?

Dear all, I have extracted RNA from urine samples using the Phenol-Chloroform extraction method and obtained the yield (~83ng/uL) on Quantifluor Fluorometer. But I could not get a clear band on...

13 December 2020 6,578 3 View

In TGA analysis weight loss % graph after 800 degree it is going upwards?

Kindly help me to analysis my TGA weight loss % graph up to to 800 degree weight loss is decreases whereas after 800 degree weight loss increases. can some help me to understand. Thanks in advance

21 March 2020 7,884 9 View

How to remove organic molecules selectively in top surface?

22 February 2020 1,936 2 View

What is the concept of increasing convective heat transfer coefficient with increase in Renolds number?

Generally Heat transfer coefficient of fluid increases with the increase in Reynolds number or velocity of fluid when it flows through a pipe. What parameters of fluid increases that causes an...

10 February 2017 6,462 8 View

Which is a better fixative for Focus forming assay..ice cold methanol or paraformaldehyde?

Anyone have done comparison of ffu assay using ice cold methanol and paraformaldehyde? which is better in terms of getting a clear foci? Any tips to get a better number of foci?

16 November 2016 3,708 3 View

What are the scope and features of 2D transition metal dichalcogenides, to prepare by wet milling and used for energy related applications?

Graphene is not optimistic in semiconductor industry, on the other hand other 2D materials have lot of scope in it. I guess we cannot make defect free and lengthier 2D materials using wet milling....

03 March 2014 9,032 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View