CDNA synthesis or qPCR did not work?

Hi everyone! I extracted RNA from urine pellet using phenol-chloroform method. The yield of RNA was 160ng/uL. 3uL of RNA was used for cDNA synthesis in 20 uL reaction using Go-Script kit....

05 February 2021 6,587 4 View

How to improve the techniques for gel picture of RNA?

Dear all, I have extracted RNA from urine samples using the Phenol-Chloroform extraction method and obtained the yield (~83ng/uL) on Quantifluor Fluorometer. But I could not get a clear band on...

13 December 2020 6,578 3 View

Study design for monitor athletes progress?

What will be the best study design for monitoring athletes progress every 6 months for 3 years. There will be an intervention program after first data collection. No control group or randomized...

10 October 2020 7,262 2 View

In TGA analysis weight loss % graph after 800 degree it is going upwards?

Kindly help me to analysis my TGA weight loss % graph up to to 800 degree weight loss is decreases whereas after 800 degree weight loss increases. can some help me to understand. Thanks in advance

21 March 2020 7,884 9 View

What is the concept of increasing convective heat transfer coefficient with increase in Renolds number?

Generally Heat transfer coefficient of fluid increases with the increase in Reynolds number or velocity of fluid when it flows through a pipe. What parameters of fluid increases that causes an...

10 February 2017 6,462 8 View

For cloning of large PCR product (20 kb) Any recommendation ?

I need to clone 20KB PCR product into vector. Any recommendation of Vector?

08 January 2017 4,104 2 View

On which computational technique does CST Studio Suite work?

In the CST manual it is mentioned that CST works by discretising maxwell's integral equation. It is also mentioned that for CST Microwave Studio, it uses central finite difference techniques for...

28 April 2014 3,178 9 View

What are the scope and features of 2D transition metal dichalcogenides, to prepare by wet milling and used for energy related applications?

Graphene is not optimistic in semiconductor industry, on the other hand other 2D materials have lot of scope in it. I guess we cannot make defect free and lengthier 2D materials using wet milling....

03 March 2014 9,032 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to find the capacitive response frequency from the impedance plot?

I have done electrochemical impedance by using cyclic voltammetry for one organic material. from that, I plotted bode diagram. but actually, I am very confused about how to find the capacitive...

18 February 2021 3,023 3 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View