For calculation of Total imp is it right to sum the area of all imp and calculate total imp or is it right to calculate each imp and sum up for Total imp?

Tabrez Shaikh @Tabrez_Shaikh

12 December 2012 1 7K Report

Consider response factor 1.0 for all impurities

Bocancea Igor

Did u mean Total imp.=S1+S2+S3+..

or Total imp.=C(%)1+C(%)2+C(%)3+... ?

The first method is the right one: Total imp.=S1+S2+S3+.., then S(total)*100%/S(ref)

Badges
Science method

More Tabrez Shaikh's questions See All

What is the classification of models Caesar, ISS, SarPy and KNN as per ICH M7 i.e. Expert rule or statistical

Statistical or Expert rule based

05 June 2017 7,189 0 View

How to analyse Formic acid on HPLC using UV detector and detection limit below 1 ppm?

HPLC and UV detector

31 December 2012 2,822 5 View

What should be the RSD for three injections for analyte peak area of intermediate product?

Rsd

09 October 2012 3,065 7 View

What is Caesar, ISS, SarPy and KNN software classification as per ICH M7

01 January 1970 4,885 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View