Generally use 10% neutrally bufferred formalin for overnight and then do embedding and freezing
We just incubate the fat pads in 10% Buffered formalin phosphate for about 24 hrs to 48 hrs (based the size of the adipose tissue).
How much buffered formalin phosphate do you use compared to the amount of tissue?
You may soak the fat chunk in the formalin
The bigger the fat chunk the more formalin you should use. I am just having problems with fixing my tissue and I think I didn't use enough volume of formalin and I was wondering if you use a certain volume / tissue.
Thanks for your feedback.
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