There are many possibilities. Your choice may depend on how much you can afford to spend and how you want to attach the dye. One good place to look is the Molecular Probes catalog.
http://www.lifetechnologies.com/us/en/home/brands/molecular-probes.html
Yes, you have to clarify how you will attach your acceptor. Another point is did you measure your donor lifetime? It is also important for FRET. You can use C153 as acceptor. Besides, there are several others which absorb this region.
Another important consideration is the actual distance range you anticipate - this information will help you to pick an acceptor with the appropriate overlap integral. For example if you anticipate a distance in the range of 40 angstrom you would prefer a donor-acceptor pair with a Forster Critical Transfer distance near 40 angstroms. You can find tables in the literature listing these distances for many donor-acceptor pairs.
Dear David Thanks for information but can you help me with the tables you have mentioned i do not get those in my search probably our institution access is limited.
kindly favor with any such tables from literature.
The 1994 book "Resonance Energy Transfer" by Vandermeer, Coker and Chen list hundreds of donor-acceptor pairs. If your institute does not have it perhaps you can get it from an interlibrary loan. Otherwise there are many web sites that list many pairs. For example: http://www.olympusmicro.com/primer/techniques/fluorescence/fret/fretintro.html
http://chemwiki.ucdavis.edu/Theoretical_Chemistry/Fundamentals/Fluorescence_Resonance_Energy_Transfer
http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence