Hi all scientists,

I am going to determine the LIVE/ DEAD fungi and bacteria cells with fluorescent dye in the possible biofilm on my plastic films surface.

I have TTC (tetrazolium chloride) but I do not think this is fluorescent. I am going to order dyes and prepare it by myself ( viability kits are very expensive). Does anyone know what dye I should use to be suitable for both bacteria and fungi. Is the protocol and method. In my mind FDI (fluorescein diacetate) for staining membrane and Pi (propidium iodide) for staining nucleus.

Many thanks in advance

if anyone has done it before or have a nice protocol please share it with me.