I am trying to plan an experiment in which I am trying to silence neurons in a circuit-specific manner. I would like to target neurons in a certain nucleus that project to the cortex and synapse onto neurons that descend to the spinal cord. I'm looking for either an anterograde AAV or a mouse that expresses DREADDs (particularly hmd4i) in a Flp-dependent manner. I've found mice that express DREADDs but require both Flp/Cre recombinase, but because of my experiment design (I don't want to burden you with details) I can't express Cre in my neurons of interest. Any ideas? Thanks!
I currently breed mouse lines that express VGaT::FlpO, VGluT2::Cre, or both. We inject DREADDS (both hM4D and hM3D) using stereotaxic techniques into the specific brain regions we are targeting. As far as I know, we are one of the only, if not the only lab, to have access to the double transgenic mouse expressing both Cre and FlpO in this fashion. I’m happy to help further if needed!
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