Hi all,
Am I looking for a Cytometry protocol for Candida Glabrata/ Yeast?
Thank you,
Ammar
Hello Ammar,
what exactly do you need the protocol for? Do you want to see the cells only in the FSC/SSC, do you want to do stainings - if so, which ones?, do you want to detect C. Glabrata, only specifically, etc.?
What kind of FACS do you have?
Best regards
Stephanie
Thank you Stephanie Kadow Stephanie,
the purpose is only to see if my transformed C. Glabrata strains that include epitopes that expressed engineering ligands.
Do you want to see the cells only in the FSC/SSC ? yes
do you want to do stainings - if so, which ones? no
Best regards,
Ammar
Hello, Ammar,
i did not understand the answer with the expressed epidopes and therefore i do not know what your read out should be. I can only give a general answer now.
If you only need FSC/SSC - have you already tried to FACS the cells? What do you see?
I mean, basically you only need to have enough cells in your sample to do the baseline settings. I.e. set FSC/SSC settings (if necessary reduce the threshold, because the cells are relatively small), so that you can see the population well.
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