Hi. I am looking for a FISH probe that does not have any fluorophore or DIG/Biotin molecule attached to it. Instead I need some inactive protein like Cas-9 (Or any other available protein) attached to it. If that's possible to do then Is it available anywhere? If do then please suggest.
Thank you.
Hi Deven,
I have never heard of probes that have Proteins like dCas9 attached to them until now. I know that you can target dCas13 to your target RNA. Just curious, why would you not want a fluorephore or DIG/Biotin attached to your probe?
Hi Sreemukta. Thank you for your interest in my question and for your reply.
I did try fluorophore attached as well as DIG/Biotin attached probes but the problem with DIG/Biotin probes is that it has lot of background signal and fluorophore attached probe does not have strong signal. So I was curious if something like I proposed in my question is available so I can use primary antibody to detect the protein attached to the probe and then do conventional assay to detect the signal.
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