Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Do I have the right PCR cycler settings?

Hello. Someone designed primers and cycler settings for me and it doesn't work. I do hot start PCR with touchdown, I detect Babesia sp. DNA in Acari. The primers are F: GYYTTGTAATTGGAATGATGG, R:...

01 March 2020 3,949 3 View

How do I determine differences between two hemp seed samples to determine if they are genetically different strains or the same strain?

Are there currentlly any methods developed for determining the differences in genome between two samples?

08 August 2019 4,594 2 View

My cells started to form clumps during passaging. I tried almost everything to reduce them. Do you have any suggestions how to solve this problem?

My cell cultures (HeLa S3, mouse kidney cells, WB-neo, WB-ras) strarted to form clumps during passaging so it is not possible to count them properly. I TRIED 1. make PBS without Mg and Ca...

14 January 2019 9,398 6 View

How to remove the Percoll from the sample?

I use density gradient centrifugation with Percoll to extract bacteria from the bentonite sample. It is working quite well, but I need to remove the Percoll from the bacterial extract at the end...

25 July 2018 981 3 View

How influence the concentration of oxygen growth of oxide layer and properties of oxide layer itself due to thermal oxidation?

Hello, I'm working on my diploma work on thermal oxidation of TiNb alloy.In my work I have oxidized titanium by two procedures thermal oxidation in laboratory atmosphere and in atmosphere of...

22 May 2018 1,704 5 View

I'm looking for taxonomic keys (for genera) - for identification of grasshoppers from Borneo (Malaysian part). Please, do you know some keys?

01 March 2016 6,885 5 View

Does anyone know whether a chromatography mode exists when both the stationary and mobile phase (developing solvent in TLC) are nonpolar?

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10 October 2012 8,485 11 View

Stimulation of CD8+ CTL with specific antigen in vitro

How to increase effectivity of CTL stimulation with synthetic peptide epitopes.

13 October 2011 2,226 1 View

Nominal vs actual concentration exposure of drugs to aquatic organisms

Hello, Do you know where I can find any critics on ecotoxicological studies which are focused on exposure aquatic organism to drugs? I am looking for papers that critic actual exposure...

01 January 1970 3,981 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View