I have 4 X 10 E10 PFU of my viral stock and I have to follow a protocol for the viral infection which suggests a MOI of 0.01, diluting the virus in 40 mL of BHI for infection per O157:H7 (containing 1.5 X 10 E8 cells). I tried to make calculations but I'm not sure they are correct. How many microliters do I have to use from my viral stock?

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