Hello all,
I'm performing the digestion of milk samples through the Infogest protocol to use it on a cell culture afterwards.
Would it be advisable to filtrate the digesta to avoid osmotic shock? And what would be the best membrane pore size? I've read you can dilute it to a physiological value but I wouldn't like to dilute it so much.
Thanks in advance, Evelyn.
For approximately 1g of sample (any biological tissue sample including milk): Add 5 ml of concentrated HNO3. Reflux for 1.5- 2 hours at 92-95 C without boiling or drying. Check the sample digestion status: If the sample is not totally digested, add another 3ml HNO3 or more until the samples all dissolved in the acid. https://www.researchgate.net/post/How_do_you_digest_cow_milk_samples_for_heavy_metals_analysis
Kindly check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010228/
Also check https://pubs.rsc.org/en/content/articlelanding/2014/ja/c3ja50330e#!divAbstract
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