Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

What fluorescent dye can be used in fixed cells for membrane imaging?

07 January 2021 4,802 2 View

Morphogeometrics analyses: how do I know which landmark has the greatest variance?

I'm using MorphoJ, and the software gives me the eigen values but it doesn't give you the eigen vector. For each landmark it gives you a x and y value for each PC but I'm looking for a value that...

21 December 2020 3,466 3 View

How to calculate Dose rate \dot {D} without source activity with dose tally * F8?

I am using MCNP5 to calculate the dose in a Brachytherapy source with dose counter * F8, but I do not have the current activity of the source. How can I calculate the dose rate for this? Thanks...

06 November 2020 4,194 3 View

Methods for bird trend analysis?

What would be the best method for analysing trends in species abundance in a single area in Latin America over a 15 year period? Could TRIM be used for this purpose? I don't have data about...

27 October 2020 7,532 5 View

What do you think about social exclusion?

The social exclusion is a problem very important for society, we should all help those most need

12 March 2020 1,152 3 View

PJET ligation not working?

I am trying to clone a 1.3 kb fragment using the pJet system. It is a sticky end cloning protocol and I have tried different ratios (from 3:1 to 8:1). I have used DH5a and Top10 cells with...

18 February 2020 9,178 3 View

How can I normalize Western blots if housekeeping proteins change in time?

Hello, I am evaluating the expression pattern of some proteins during the process of neuronal differentiation (1 day in vitro to 32 days in vitro) in primary neuron culture using western blot. I...

08 September 2019 1,505 3 View

Does anybody have the lastest version of the "Handbook of the Birds f the World"?

I have a copy but it is from 1992, and I need an updated one. I just need the section about the Procellariiformes, the Systematics. You can also suscribe online to the webpage...

09 July 2019 4,090 2 View

How to extend the life shell of a biofertilizer?

Hi I am currently working in a project to develop a biofertilizer but my main problem is that my product only last 1-2 weeks and then it gets rotten. Is there any protocol or conservative sustance...

27 January 2019 9,096 4 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View