Hello, I prepared 4 mg/mL corticosterone in sesame oil for subcutaneous injection in mice. To do so, I had to sonicate and warm up the solution to facilitate corticosterone solubility. I was wondering if I could sterilize the solution before administration and how I can do that. I am worried I could cause infections at the injection site. I can't filter because sesame oil is very thick. I appreciate any feedback. Thanks
[Not a biochemist speaking]: unraffinated sesame oil can be heated up to anything below its smoke point of 177°C, that should be enough to eliminate all lifeforms from your solution.
Corticosterone has a melting point of 181°C, so it seems that the component on its own should also withstand a sterilizing temperature.
However, you can't exclude that trace components of the oil will react with corticosterone at higher temperatures. Would it be possible to thermally sterilize both components separately and perform the mixing while keeping it all clean?
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