Has anyone performed stimulation of fibroblasts to myofibroblasts in cell lines. I found some articles on primary cultures. does the same work with cell lines?
Ash Sargent
Midgley et al., 2013. Just use 10ng/mL TGFB for about 24 hours and it should be fine.
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Aggelos Vrynas
TGFb (5ng/ml) works, but can be also fibroblast origin dependent. IL-4, IL-13 and TNF (all at 10ng/ml) also work.
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