Dear all,
I am looking for a myofibroblast surface marker antibody (preferably not Mouse) that can be used to identify fixed, non-permeabilised fibroblast cells during flow cytometry?
I have tried using 'Anti-fibroblast surface marker protein' from Sigma but have had mixed results.
Any other suggestions would be extremely helpful.
Thank you very much in advance
Hi Dominic, fibroblasts are not a specific cell type like endothelial cells, smooth muscle cells, etc.
They are collectively called fibroblasts because they look similar when they are adherent. They can include keratinocytes, pericytes, certain epithelial cells, mesenchymal cells, etc. So there is no universally accepted specific cell surface markers for "fibroblasts".
If you can narrow the specific function that you are looking for in your cells then you can probably find a marker for it.
Hi Dominic, fibroblasts are not a specific cell type like endothelial cells, smooth muscle cells, etc.
They are collectively called fibroblasts because they look similar when they are adherent. They can include keratinocytes, pericytes, certain epithelial cells, mesenchymal cells, etc. So there is no universally accepted specific cell surface markers for "fibroblasts".
If you can narrow the specific function that you are looking for in your cells then you can probably find a marker for it.
This thread from 4 years ago looks interesting for you: https://www.researchgate.net/post/What_are_the_best_alternatives_to_Vimentin_as_a_Fibroblast_Marker
Hello
Yes i agree with Steingrimur Stefansson , there are not specific cell surface markers for ( fibroblasts ) . please define your cell type to help you .
https://www.ncbi.nlm.nih.gov/pubmed/28383825
Good Luck
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