I am interested in Feline retroviruses, specifically FeLV, Is any member on the same tract?
We are interested in drawing a model of a nanoparticle-cell interaction using cartoon diagram. Any free software available or assistance?
08 September 2014 6,822 1 View
We are dealing with an unknown/yet to be identified virus suspected to belong to chicken coronavirus [infectious bronchitis virus]. There was a history of high mortality and evidence of kidney...
07 August 2013 2,790 13 View
We have performed negative staining of DNA sample using the PTA staining method and to our surprise no sample was observed under TEM. We also need to visualize the DNA when incorporated in a...
07 August 2013 9,050 3 View
I am interested in detecting two closely related genes expressed in mammalian cells. These genes are likely to react with same primary antibody. I thought of tagging the first gene with...
07 August 2013 5,248 6 View
Q1: I used several methods reported in different journals to prepare 1% chitosan nanoparticle in 1% acetic acid . In the final, I intend to use about
05 June 2013 4,872 5 View
Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...
07 May 2024 259 3 View
Hi follow virologists, I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased...
18 March 2024 5,174 4 View
Dear virologists; What is the PCR technique used in virology to detect viral nucleic acids? The steps involved. I'd also like to know, since some viruses have a single strand of DNA and RNA, how...
28 February 2024 1,869 10 View
Is it possible that a hybrid virus is generated in a eukaryotic cell by co-infection of different viruses through nonhomologous recombination?
14 September 2023 7,245 3 View
For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...
22 August 2023 1,600 1 View
I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.
26 July 2023 7,550 1 View
Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...
29 May 2023 7,105 6 View
Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...
17 April 2023 5,873 6 View
I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...
12 January 2023 4,248 2 View
Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.
27 November 2022 9,614 2 View