We have been culturing cells for years now, but we have recently been observing a strange behavior, especially in cell lines like MCF-7, CaCo2, HepG2 and L929, that they are detaching from the T-flasks post 2 to 3 days. We change the media every alternate day, with the composition as per ATCC recommendations. This behavior has affected our workflow immensely. Please find the details below:
Media make: HiMedia (FBS we use is heat inactivated)
T-flasks make: Tarsons
Incubator: Water jacketed 5%CO2 thermo scientific
What we are suspecting is there might be an issue with the CO2 value displayed and thus we have requested for breakdown visit. Additionally, we are also identifying T-flasks make as one of the probable reasons. Any leads will be appreciated.
Are RPMI-1640 medium and the new Thermo-Fisher t Flasks affordable?
Dear Sundus,
They are relatively expensive as compared to the make we use, but affordable with the project at stake.
Dear Chintan
That is true, they are expensive but the best to perform experiments. Try to take out new cells from the liquid nitrogen and started your experiment again. Make sure that your t flasks are for adherent cells.
Best wishes.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line