How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why does my freeze-dried culture of Carboxydothermus hydrogenoformans (DSM6008) not revive?

I have tried to revive the above organism from a freeze dried culture provided by DSMZ. As is usually the practise, instead of utilizing the entire culture at one go, we use sterile loops to...

01 February 2019 7,970 12 View

How would you make the Agar plates at pH 2-3 for the extremophiles organism?

Respected Seniors! I am having a problem at making the agar plates at pH 2-3. The reason is hydrolysis of agar at that pH during sterilization. Any positive suggestion. Thank you

14 April 2018 2,040 5 View

How would you make the Agar plates at pH 2-3 for the extremophiles organism?

Respected Seniors! I am having a problem at making the agar plates at pH 2-3. The reason is hydrolysis of agar at that pH during sterilization. Any positive suggestion. Thank you

03 April 2018 4,817 6 View

Is there any evidence about toxicity of Aphanius flesh/organs when eaten by predator?

An old paper by White et al. (1965) describes the toxicity of two species of Fundulus (Cyprinodontiformes, Fundulidae) to some warm-blooded vertebrates (birds and mammals). Despite some...

08 September 2017 5,343 5 View

Could thermophilic bacteria that have been preserved in glycerol solution at -80 C for a year loss their ability to grow at high temperature?

I have 5 thermophilic bacteria that had been preserved for a year in glycerol solution (15%) at -80 oC. I tried to grow the five isolates in LB broth, 50 oC, 150 rpm, but only 3 isolates grew...

16 August 2017 1,783 3 View

Can we put intracellular pathogens in the group of extremophiles?

Intracellular pathogens are able to survive inside macrophage where pH reached up to 2 inside phagolysosome. Is this fine if we can put these pathogens that have developed mechanism to survive in...

13 August 2017 2,276 3 View

Can we put intracellular pathogens in the group of extremophiles?

Intracellular pathogens are able to survive inside macrophage where pH reached up to 2 inside phagolysosome. Is this fine if we can put these pathogens that have developed mechanism to survive in...

07 August 2017 4,339 4 View

Can any one help me in knowing some important tools/software used in Metabolic Engineering and Bioinformatic studies?

I am interested to study Metabolic engineering and Bioinformatic subject. I want to work on the molecular mechanisms of the microbes found in extreme environments (extremophiles). In this regard,...

07 August 2017 2,217 7 View

Could thermophilic bacteria that have been preserved in glycerol solution at -80 C for a year loss their ability to grow at high temperature?

I have 5 thermophilic bacteria that had been preserved for a year in glycerol solution (15%) at -80 oC. I tried to grow the five isolates in LB broth, 50 oC, 150 rpm, but only 3 isolates grew...

07 August 2017 7,154 4 View

Can any one help me in knowing some important tools/software used in Metabolic Engineering and Bioinformatic studies?

I am interested to study Metabolic engineering and Bioinformatic subject. I want to work on the molecular mechanisms of the microbes found in extreme environments (extremophiles). In this regard,...

04 August 2017 7,979 5 View