Rishi Gandhi

6 Questions 5 Answers 0 Followers

Questions related from Rishi Gandhi

Can smear be removed by PCR cleanup or is there any PCR cleanup protocol to enhance the result?

I will be using the Himedia PCR cleanup protocol and kit, so if anyone has any hacks to reduce the smear, do let me know. I will be sending samples urgently for sequencing. The sequencing is...

04 January 2024 6,596 3 View

DNSA method gives the same result in Test and control, so what can be the reason?

We grow the microbe in complex sugar media, spin it, take the supernatant, and add buffer and water. In the case of a test, we add the substrate and incubate it before adding DNSA, bringing it to...

06 October 2023 6,281 0 View

Plate confirmatory method to find Xylose utilizing microbes?

We have tried TTC (Tri Methyl tetrazolium chloride) in NaOH on xylose plate TTC directly added to Xylose media and I am not getting any results. Please help me out.

06 June 2023 7,378 5 View

Nonspecific PCR products and faint bands with Q5 polymerase (0for overlapping PCR)?

I am currently using Q5 polymerase from NEB to amplify a protein of 2.5 Kb. I have designed the primers to target the 21 BP that are compliment to the DNA sequence , the restriction sites are...

01 May 2019 9,791 3 View

Extremophile vs Extremophilic microbes

Could anyone please let me know the differences between Extremophile vs Extremophilic microbes. I would highly suggest to link the paper from where you defining the differences.

01 January 1970 8,841 4 View

Plate confirmatory method to find Xylose utilizing microbes.

We have tried TTC (Tri Methyl tetrazolium chloride) in NaOH on xylose plate TTC directly added to Xylose media and I am not getting any results. Please help me out.

01 January 1970 1,689 0 View