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Questions related from Rishi Gandhi
Dear Colleagues, I am currently working on genomic DNA extraction from mangrove soil using the NucleoSpin Soil Kit (Takara Bio), but I am facing issues with low DNA yield, No DNA on gel, no PCR...
28 February 2025 9,403 2 View
We sought to report the genus diversity of regions and connect it with some other characteristics and enzymes. The last batch had a lower quality value (20+), but the majority had an average base...
19 July 2024 3,706 0 View
I will be using the Himedia PCR cleanup protocol and kit, so if anyone has any hacks to reduce the smear, do let me know. I will be sending samples urgently for sequencing. The sequencing is...
04 January 2024 6,675 3 View
We grow the microbe in complex sugar media, spin it, take the supernatant, and add buffer and water. In the case of a test, we add the substrate and incubate it before adding DNSA, bringing it to...
06 October 2023 6,350 0 View
We have tried TTC (Tri Methyl tetrazolium chloride) in NaOH on xylose plate TTC directly added to Xylose media and I am not getting any results. Please help me out.
06 June 2023 7,429 5 View
I am currently using Q5 polymerase from NEB to amplify a protein of 2.5 Kb. I have designed the primers to target the 21 BP that are compliment to the DNA sequence , the restriction sites are...
01 May 2019 9,881 3 View
Could anyone please let me know the differences between Extremophile vs Extremophilic microbes. I would highly suggest to link the paper from where you defining the differences.
01 January 1970 8,890 4 View
01 January 1970 1,729 0 View
