Which is the best protocol to extract periplasmic protein expressed in deep well plate.
Em relação aos empregos que estão sendo substituídos pela inteligência artificial, como está sendo organizada a formação e a realocação dos colaboradores? Pois muitos empregos ficarão escassos, e...
31 July 2024 6,864 1 View
I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...
01 July 2024 10,003 1 View
i am working on a project where i need to find a protocol that shows how to quantify cultured cells inside a stirred tank bioreactor but every where i searched i found nothing
23 June 2024 5,687 3 View
Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...
30 May 2024 7,431 3 View
nano additives and lubricants for hybrid electric vehicles
24 May 2024 8,363 3 View
Good afternoon everyone, I am starting to perform cell culture of spheroids and I am encountering some doubts and difficulties. After using the methodology of 0.4% methylcellulose + DMEM HIGH...
20 May 2024 2,363 0 View
Please share your ideas and suggestions regarding that "Exogenous application of melatonin reduces autophagy activity in plants under drought stress conditions". If someone knows the phenomenon...
20 May 2024 6,561 1 View
Assuming this is my hypothetical data set (attached figure), in which the thickness of a structure was evaluated in the defined positions (1-3) in 2 groups (control and treated). I emphasize that...
09 May 2024 871 4 View
ΔG = 12.77 ΔH = -37,92 ΔS = -162,40
07 May 2024 4,942 3 View
hey, is there anyone who used irradiations for high entropy alloy at room temperature ?
28 April 2024 1,824 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View