Lucas F. Ribeiro

6 Questions 4 Answers 0 Followers

Questions related from Lucas F. Ribeiro

What are the possible effects of DTT or β-mercaptoethanol, unrelated to thiol disulfide exchange, on protein activity?

I have seen on literature some purified glycoside hydrolases, without cysteines, that showed their activity increased by addicition of DTT or β-mercaptoethanol on enzimatic assay. What are the...

23 February 2017 282 3 View

Does someone know a good online tool to generate 3D DNA structure (PDB model) using as an input a DNA sequence?

I'm looking for a good server for creating DNA 3D structure based on small sequences.

03 February 2017 2,544 3 View

Is MODELLER able to process circular permutations?

I have produced a number of circular permutations for a protein. There are several structures available for the wild type protein. Is MODELLER able to process circular permutations? If so, how...

17 November 2015 9,098 1 View

Does anyone know which mechanism(s) deficient RecA E. coli use to make deletions/recombination in a plasmidial gene and how to avoid it?

I have a library with variants of the same gene, and after selection steps (antibiotic resistance) I have observed a large number of variants with deletions. I don't have these deletions in my...

04 March 2015 1,518 3 View

What are the techniques involved in the directed evolution of enzymes?

28 November 2013 4,472 3 View

Extraction of periplasmic protein

Which is the best protocol to extract periplasmic protein expressed in deep well plate.

06 October 2012 565 1 View