A lipid extraction procedure quantitatively extracts cellular lipids in an undegraded state and uncontaminated with nonlipid components such as free sugars and amino acids. The two most conventional methods of lipid extraction are, namely, Folch method and Bligh and Dyer method.Enveloped viruses acquire lipid membranes as their outer coat through interactions with cellular membranes during morphogenesis within, and egress from, infected cells. In contrast, non-enveloped viruses typically exit cells by cell lysis, and lipid membranes are not part of the released virions. Viral envelopes consist of a lipid bilayer that closely surrounds a shell of virus-encoded membrane-associated proteins. The exterior of the bilayer is studded with virus-coded, glycosylated (trans-) membrane proteins. The main component of the viral envelope is the host-derived lipid bilayer. The precise composition of this lipid membrane varies, as different viruses acquire their envelopes from different cellular membranes. The virus envelope is known as a capsid. Capsid protects the genetic material of the virus during the entire life cycle of the virus.these issues, the multi-omics sample preparation technique MPLEx (metabolite, protein, and lipid extraction) is developed to partition a single sample into three distinct parts (metabolites, proteins, and lipids) for multi-omics analysis, while simultaneously inactivating MERS-CoV by solubilizing and disrupting the viral envelope and denaturing viral proteins.