Unicellular green algae are a source of auxins, how can I extract them? Furthermore, I'm looking for an certifiably organic technique.
Dear Karin,
Here are few papers for your reference.
1. WILLIAM P. JACOBS, KATHY FALKENSTEIN, AND ROBERT H. HAMILTON,Control of cytokinin and auxin homeostasis in cyanobacteria and algae,Plant Physiol. (1985) 78, 844-848
2. Šimura JNovák OStrnad MNedbal L. 2014. Cytokinin profiling in Cyanobacteria and microalgae species using UHPLC-MS/MS. In: Abstracts of the International Symposium 2014: Auxins and Cytokinins in Plant Development … and Interactions with Other Phytohormones, Prague, Czech Republic. Abstract P1–15.
3. Eva Žižková, Martin Kubeš, Petre I. Dobrev, Pavel Přibyl, Jan Šimura, Lenka Zahajská, Lenka Záveská Drábková, Ondřej Novák, Václav Motyka; Control of cytokinin and auxin homeostasis in cyanobacteria and algae. Ann Bot 2016; 119 (1): 151-166.
Good luck!
https://academic.oup.com/aob/article/119/1/151/2670029/Control-of-cytokinin-and-auxin-homeostasis-in
you could grow the algae on pure culture, and then take 50ml of the pure culture, change the pH to 2.5 and extract four times with freshly distilled di -ethyl ether four times, combine the extract, evaporate using rotary evaporator at 35C to 2ml, then HPLC for IAA.
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