How to prepare a Gaussian input file for a binary solvent?

The UV absorption energy spectrum of a molecule has been recorded experimentally using binary solvent (CH3Cl:CH3OH =10:1 v/v). I am trying to calculate the absorption energy of the same molecule...

15 March 2020 9,396 3 View

How can i track a mining area through satellite imagery (Sentinel 2 for example) ?

I'm trying to visualize the changer in a mining area over time (the new buildings, the exploitation ..) and I can't find enough documentation, I prefer to do this work in python since I'm familiar...

15 January 2020 373 7 View

Does anyone have experience with DNA extraction from formalin fixed paraffin embedded (FFPE) tissue?

I am using QIAamp DNA FFPE Tissue Kit for DNA extraction; i followed the manufacturer protocol but i've got a smeer, what should i do. thank you for your help

05 February 2018 3,207 4 View

How we can do correction of abnormal pixels in Hyperion images using ENVI or ERDAS softwares ?

How we can do corrections of abnormal pixels in Hyperion images using ENVI or ERDAS softwares ? We do it before geometric corrections ? Before classification ? or we can do it after...

04 February 2018 7,381 2 View

How do I perform atmospheric correction in EO-1 Hyperion data? how i can remove the noisy bands and eliminate also the bad line ?

I need to remove the noisy bands of Hyperion EO1 image , i downoald the image from earthexplorer.usgs.gov as 242 seperate bands. and what is the good method in ENVI 5.3 for doing athmospheric...

28 January 2018 9,454 3 View

What is the best LPS-dose to induce chronic systemic inflammation to mice ?

Hi, I am starting a project that requires to induce a chronic systemic inflammation to mice. I try to figure out which dose of LPS I can give to mice and maybe how many times I should give them to...

21 January 2018 3,648 6 View

Resampling remote sensing image for image fusion ?

i have a hyperspectral image with 3m spatial resolution and have multispectral image with 1.6 m spatial resolution. I want to resample the hyperspectral image resolution to 6.4 m after i should...

18 January 2018 1,797 15 View

What is the effect of organic solvents on DNA conformation?

does the strucure of DNA will be conserved in organic medium?

31 October 2017 4,974 5 View

Matching between insilico PCR product size and agarose gel resultas after PCR reaction?

31 January 2017 6,987 4 View

Can any one tell me what can it be ?

found in the stomach of Squilla mantis (Algeria)

29 June 2016 8,641 7 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View