Extract tight gap junction proteins?

01 October 2024 1 7K Report

I want to extract tight gap junction proteins from the colon cell-cell junction, excluding the cytosolic fraction. Is there any technique available except subcellular fractionation kit by Thermofischer?

Malcolm Nobre

Hello Sangita Ghimire

Please refer to the article attached below. See Fig 3 for the protocol, which provides different stages of biochemical purification of tight junction complexes.

Article Proteomic and bioinformatic analysis of epithelial tight jun...

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