Sangita Ghimire

3 Questions 1 Answers 0 Followers

Questions related from Sangita Ghimire

Growing Caco-2 cells in coverslips for 21 days?

Hi, I am growing Caco-2 cells on PDL coated coverslips for immunocytochemistry. Most literature mention the cells require 21 days to differentiate fully. However, I’ve noticed that my Caco-2 cells...

17 January 2025 7,177 0 View

Primary antibody to label caco2 cell membrane?

I am planning to label Caco-2 cell membranes for my experiments and would appreciate recommendations on the best primary antibody for this purpose. I am looking for antibodies that specifically...

12 December 2024 4,548 1 View

Extract tight gap junction proteins?

I want to extract tight gap junction proteins from the colon cell-cell junction, excluding the cytosolic fraction. Is there any technique available except subcellular fractionation kit by...

01 October 2024 7,242 1 View