I am looking for data comparing expression in liver using a CBh promoter to expression using either CMV or CAG in an AAV vector. Any help would be appreciated.
Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...
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TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,483 1 View
I found the following recipe at Abcam and converted it to 5x to make a stock solution. Running buffer (Tris-Glycine/SDS) 25 mM Tris base 190 mM glycine 0.1% SDS Check the pH and adjust to...
31 May 2024 1,444 2 View
Who of all of you that see, read this will make action' with me to save this world from its NOW happening global thermodynamics of of a very bad exterminating start of 01/05/2047, and into a...
09 March 2024 1,040 18 View
I am looking for datasets to supplement the NHANES datasets.
09 February 2024 1,471 0 View
Looking to perform a non-radioactive EMSA to study protein binding to ssDNA. Before doing so, I’m looking to optimize detection. The two detection series are: 1) biotinylated oligo, strep-HRP...
01 February 2024 8,161 0 View
Dear colleagues and members, the researchgate member Budee U Zaman (researchgate.net) copied one of my preprints one-to-one and passed it off as his own work. He only slightly changed the title....
04 January 2024 4,312 1 View
Good day! I am trying to find a way to measure hesperidin content of the peels of my fruit sample (Citrus reticulata), other than HPLC. I plan to ferment my peels with food-derived mixed cultures...
13 October 2023 1,249 2 View
Can you compile a calibration curve with a standard that contains various compounds? For example a fatty acid methyl ester mix given that you know the concentration of each fatty acid in the mix?...
03 October 2023 2,109 1 View
Anyone know of a buffer that will work adequately for PCR with Takara's Primestar GXL? I have tons of enzyme but am out of buffer. Takara will sell a pack of extra buffer tubes, but it won't...
12 September 2023 2,451 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,114 1 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,481 3 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 780 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,667 5 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,176 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,642 3 View