Mike Mcdonald

4 Questions 2 Answers 0 Followers

Questions related from Mike Mcdonald

Can I put a WPRE upstream of IRES in a bicistronic vector?

I am interested in creating a bicistronic vector in which the WPRE follow the first gene and is upstream of the IRES rather than following the second gene and downsteam of the IRES. Can you...

07 August 2023 8,488 0 View

expression in liver using a CBh promoter compared to expression using either CMV or CAG in an AAV?

I am looking for data comparing expression in liver using a CBh promoter to expression using either CMV or CAG in an AAV vector. Any help would be appreciated.

22 August 2019 7,216 0 View

Formulation of entacapone for intraperitoneal injection in mice?

I need to formulate entacapone for i.p. administration to mice. The formulation must NOT include cyclodextrin. What do you recommend to get good blood levels

30 May 2019 593 4 View

AAV vector design for gene therapy?

I am familiar with bicistronic AAV gene therapy vectors that have two cistrons with an IRES between them. Since this is known to result in less efficient expression of the second gene why not...

16 December 2016 1,940 4 View