Hello everyone.
I want to express a yeast protein in E. coli. Should I have to optimize codon usage when express in E. coli? In case of a long gene, how can I optimize the codon usage?
Thank you so much.
Hi everyone. I would like to measure PKA activity in the yeast cell. Could you give me any suggestions? Thank you!
05 June 2016 3,869 0 View
Hello everyone, I am going to analyse some protein targets in stationary phase in some mutants. However, the mutants grow slowly, so I want to use some indicative proteins to determine the...
08 September 2015 7,607 2 View
Hello everyone, I wanna attach my cells on to the slide, so I used poly-lysine to coat the slide. I made poly-lysine solution in ddH2O with concentration was 1mg/ml and stored at -20oC. After...
01 February 2015 1,389 6 View
Dear everyone, I am going to express a portion of a protein which is about 8kDa in E. coli. I am wondering if it is hard to express small protein (
31 December 2014 7,120 6 View
Dear everyone, I want to tagged GFP to my target protein. Do I have to insert a simple peptide such as GGGG between GFP and the protein? And how many amino acids is enough? Thanks you so much.
11 December 2014 7,186 8 View
Hi everyone, I am working with BY4741 background but it seem to be not working well. So I want to change to another background such as CML128,... But I do not know much about the other backgrounds...
11 December 2014 1,469 0 View
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04 May 2013 6,158 9 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View