8 Questions 10 Answers 0 Followers
Questions related from Long Duy Duong
Hi everyone. I would like to measure PKA activity in the yeast cell. Could you give me any suggestions? Thank you!
06 June 2016 3,832 0 View
Hello everyone, I am going to analyse some protein targets in stationary phase in some mutants. However, the mutants grow slowly, so I want to use some indicative proteins to determine the...
09 September 2015 7,584 2 View
Hello everyone, I wanna attach my cells on to the slide, so I used poly-lysine to coat the slide. I made poly-lysine solution in ddH2O with concentration was 1mg/ml and stored at -20oC. After...
02 February 2015 1,356 6 View
Hello everyone. I want to express a yeast protein in E. coli. Should I have to optimize codon usage when express in E. coli? In case of a long gene, how can I optimize the codon usage? Thank you...
02 February 2015 1,746 0 View
Dear everyone, I am going to express a portion of a protein which is about 8kDa in E. coli. I am wondering if it is hard to express small protein (
01 January 2015 6,899 6 View
Hi everyone, I am working with BY4741 background but it seem to be not working well. So I want to change to another background such as CML128,... But I do not know much about the other backgrounds...
12 December 2014 1,444 0 View
Dear everyone, I want to tagged GFP to my target protein. Do I have to insert a simple peptide such as GGGG between GFP and the protein? And how many amino acids is enough? Thanks you so much.
12 December 2014 7,151 8 View
...
05 May 2013 6,133 9 View
