8 Questions 10 Answers 0 Followers
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Hi everyone. I would like to measure PKA activity in the yeast cell. Could you give me any suggestions? Thank you!
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Hello everyone, I am going to analyse some protein targets in stationary phase in some mutants. However, the mutants grow slowly, so I want to use some indicative proteins to determine the...
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Hello everyone. I want to express a yeast protein in E. coli. Should I have to optimize codon usage when express in E. coli? In case of a long gene, how can I optimize the codon usage? Thank you...
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Dear everyone, I am going to express a portion of a protein which is about 8kDa in E. coli. I am wondering if it is hard to express small protein (
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Hi everyone, I am working with BY4741 background but it seem to be not working well. So I want to change to another background such as CML128,... But I do not know much about the other backgrounds...
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Dear everyone, I want to tagged GFP to my target protein. Do I have to insert a simple peptide such as GGGG between GFP and the protein? And how many amino acids is enough? Thanks you so much.
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