Hi everyone.
I would like to measure PKA activity in the yeast cell. Could you give me any suggestions? Thank you!
Hello everyone, I am going to analyse some protein targets in stationary phase in some mutants. However, the mutants grow slowly, so I want to use some indicative proteins to determine the...
08 September 2015 7,607 2 View
Hello everyone, I wanna attach my cells on to the slide, so I used poly-lysine to coat the slide. I made poly-lysine solution in ddH2O with concentration was 1mg/ml and stored at -20oC. After...
01 February 2015 1,389 6 View
Hello everyone. I want to express a yeast protein in E. coli. Should I have to optimize codon usage when express in E. coli? In case of a long gene, how can I optimize the codon usage? Thank you...
01 February 2015 1,776 0 View
Dear everyone, I am going to express a portion of a protein which is about 8kDa in E. coli. I am wondering if it is hard to express small protein (
31 December 2014 7,120 6 View
Dear everyone, I want to tagged GFP to my target protein. Do I have to insert a simple peptide such as GGGG between GFP and the protein? And how many amino acids is enough? Thanks you so much.
11 December 2014 7,186 8 View
Hi everyone, I am working with BY4741 background but it seem to be not working well. So I want to change to another background such as CML128,... But I do not know much about the other backgrounds...
11 December 2014 1,469 0 View
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04 May 2013 6,158 9 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...
05 August 2024 2,141 4 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View