How to analyse GC MS spetra?

Kindly help to interpret GC-MS of plant extract? how to relate a particular compound from the spectra. What is important point to consider? Thank you all

09 October 2018 7,321 5 View

What gene will be suitable for molecular Plant identification?

Dear researcher, I would like to identify and confirm plants by molecular method. So, what gene will be best suited for this purpose?

08 September 2018 5,134 0 View

Can anyone suggest AntiCancer research approach?

Dear Experts, I would to carry out a research on Plant source medicinal or ethno-pharmaceutical in vitro and in vivo. I would to focus on Biochemical-Physiological profiling and molecular...

06 July 2018 7,448 0 View

Can cancer cells from animal origin develop in human?

Can cancer cells from animal origin growth, develop and proliferate in human? What if not, what exactly is the reason? is it immune system incompatible alone for not proliferation? Thanks

07 August 2016 6,356 1 View

Can ulcer be induced in short time by chemical?

Can ulcer be induced and treated well in hours? As far my understanding is concern, it is chronic symptom. Does the healing of ulcer involves division of cell or tissue formation? Thanks

07 August 2016 1,726 1 View

Is it sufficient anova way statistical analysis?

Dear researchers, I need to perform biochemical experiment data to analysis statistical significance. I have more two groups for all experiments. But I am not sure what statistical parameter will...

03 April 2016 1,818 3 View

Can anyone share me some information on western blot?

Respected Researchers, I would like to know what is the principle behind the Western blotting? How will be the expression of interest protein if treated cancer cell with drugs? I read many...

03 April 2016 3,759 2 View

Kindly help me with western blotting?

Researchers, can anyone suggest me western blotting technique? I need to perform western blotting but i have no practical experienced yet. How to develop the band or expression of protein on...

02 March 2016 3,458 6 View

What stastistical parameters will be appropriate for biochemical assays?

02 March 2016 1,113 6 View

How acurate is Acridine orange/ ethidium bromide detection of apotosis and micronuclei?

I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate. Can anyone share me...

01 February 2016 6,592 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View