Does anybody have any experience with the PGA Screen from Molecular Dimensions? Could you share yourstories?
I did some work related to isolation of a plant protein and also evaluated crystallographic parameters to characterize it.
If you are interested to know further pl. don't hesitate to contact me.
I want to inject a Cre dependent virus to be expressed in pyramidal neurons, and I have Emx1Cre adult mice. I know Emx1 is a developmental gene, but found a paper showing expression of Emx1 also...
28 March 2024 1,666 0 View
I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...
12 February 2024 7,730 1 View
Good evening, Can you please advice on a protocol for labeling of OMVs for cellular uptake assay. OMVs are derived from gram-negative strain. I was thinking of applying R-18 but other dyes are...
03 October 2023 2,929 3 View
Hello, in our lab we use F-AAS for determination of Mg in urine and Li, Zn in serum. Except Zinc QC 2 everything works fine. Is it possible there is problem with zinc controls? I am waiting for...
04 September 2023 4,169 1 View
Hi! Im experiencing problems with CamKII immunostaining for free floating mouse PFC and Hp slices. I ve tried different blocking agents ( NDS, NGS, BSA in different percentages), different mouse...
30 July 2023 7,341 0 View
I performed CRISPR-KO of p53 in fibroblasts. I've got cells that lost the activity of WTp53 (several target genes tested on qPCR) but on western blot, I see still quite a high level of a protein...
15 January 2023 8,080 0 View
How can I quantify DAB using ImageJ or QuPath (staining intensity and number of positive cells)?
03 July 2022 6,002 3 View
I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...
08 June 2022 2,467 0 View
What polymeric material has the most similar properties as nylon 6.6 in terms of the processing and mechanical properties, and it is not from the polyamides family?
05 August 2021 6,193 8 View
I am currently looking for a scale to measure online self-disclosure. The results will help me write my master's thesis.
13 July 2021 9,306 2 View
Require for Rietveld refinement in XRD.
08 August 2024 3,081 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View