Sorry i will not answer to your question, because I’m just starting working with exosomes so I still have many doubts. Instead, if you don’t mind I will ask you something:

How did you get rid of serum exosomes, the ones that are inherently in your culture medium if we use FBS.

Thanks in advance

I hope this link will help you

http://www.jove.com/video/3037/isolation-and-characterization-of-rna-containing-exosomes

Hi, in terms of getting the pelleted exosomes into solution it is for exosomes enriched using a precipitation type of protocol important to add PBS so that it covers the pellett. Thereafter, let it rest for at least 30 min at RT. Then the pellett should be easier to resolve by gentle pipetting up and down (avoid bubbles). This approach might also work for exosomes enriched by ultracentrifugation.

Hope this will help. good luck

kind regards

ketil

Ketil - Thank you for your suggestion. It worked perfectly. I actually only needed to let the pellet sit in PBS for 10 minutes at RT.

Sonia - sorry for the late reply, but serum exosomes are usually removed by ultracentrifugation, although it can be purchased from SBI (Systems Bioscience): exosome depleted FBS Cat# EXO-FBSHI-250A-, but it is pricey.