Hello everyone, I am starting to work on extracellular vesicles (EVs) so recently I did an exosome isolation from cell culture media of neuroglioma cells by using ultracentrifugation protocol. I checked the EVs size with Nanosight and obtained several pics: two major at 165 and 215 nm and two other but smaller at 355 and 535 nm (might be aggregates). Does anyone know if this profile looks like something I could expect or is that too much high for a classical EVs profile? Usually the major pic is around 100nm in accordance with literature data but it seems to be the expected profile from exosomes (a small portion of EVs from cells). Is there any cut-off I should consider more related to all type of EVs?