Can someone tell me if epperndorf biospectrophotometer with micro cuvette is really better than Nanodrop in nucleic acids quantification, even for small concentrations?
I think both gives you a same kind of values, but if you relay go for the any downstream high-through put experiment then definitely go for Qubit RNA quantification kit, in which dyes provided in the kit directly binds to the RNA but not to the DNA or protein presents in the samples and also give you a accurate and even quantify very low quantity of RNA by using Qubit HS RNA kit.
I complitely agree with Abhijit. In my experince Qubit from Invitrogen (Broad range or High Sensitivity kit) allows to quantified the DNA better than Nanodrop, expecially when the concentration is low.
I will tell you if you want very accurate in your measurement you should go for PicoGreen, even though this techniques are not as straight as Nano
Dear Karuppiah,
Check the sensitivity, reproducibility and accuracy (including stray light) of each of spectrophotometer.
Kind regards,
Ade Kujore
Marketing
Cecil Instruments Limited
Milton Technical Centre, Cambridge CB24 6AZ
United Kingdom
email:- [email protected]
telephone:- +44 (0) 1223 420821
http://www.cecilinstruments.com/bio-1.html
It's better to use nanodrop for very low quantities of the samples. but it's really necessary to have uniform concentration of the DNA or RNA that you're quantifying through out the sample stock.
I'd prefer nanodrop from thermo fisher scientific to quantify such samples. If you don't have any choice , go for the eppendorf spectrophotometer and check the optical density twice or thrice at least to make sure you get precise quantities.
Good Luck
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